Journal: Med (New York, N.y.)

Article Title: Favorable antibody responses to human coronaviruses in children and adolescents with autoimmune rheumatic diseases

doi: 10.1016/j.medj.2021.08.001

Figure Lengend Snippet: Antibodies to coronaviral spikes in pediatric and adolescent JIA, JDM, JSLE, and MIS-C patients and age-matched controls (A) Mirror plots of the specific MFI increase of HEK293T cells expressing HCoV-OC43 spike (top) or SARS-CoV-2 spike (bottom) caused by individual sera. Each bar is an individual healthy control or patient. Samples are plotted according to the signal of antibodies to HCoV-OC43 spike and in the same position in the mirror plots. Antibody levels to SARS-CoV-2 spike in MIS-C patients are plotted on a different scale from the rest. (B) Mirror plots of the specific MFI increase of HEK293T cells expressing ERV3-1 (top) or HERV-K113 (bottom) envelope glycoproteins caused by individual sera. Samples are plotted in the same order as in (A).

Article Snippet: pcCMV3- HCoV-OC43_spike , SinoBiological , Cat# VG40607-CF.

Techniques: Expressing

Journal: Med (New York, N.y.)

Article Title: Favorable antibody responses to human coronaviruses in children and adolescents with autoimmune rheumatic diseases

doi: 10.1016/j.medj.2021.08.001

Figure Lengend Snippet: Prevalence of IgG antibodies to OC43 and SARS-CoV-2 spikes in JIA, JDM, and JSLE patients.

Article Snippet: pcCMV3- HCoV-OC43_spike , SinoBiological , Cat# VG40607-CF.

Techniques:

Journal: Med (New York, N.y.)

Article Title: Favorable antibody responses to human coronaviruses in children and adolescents with autoimmune rheumatic diseases

doi: 10.1016/j.medj.2021.08.001

Figure Lengend Snippet: Antibodies to coronaviral spikes in pediatric and adolescent JIA, JDM, JSLE, and MIS-C patients and controls of different age Specific MFI increases of HEK293T cells expressing HCoV-OC43 spike (A and C) or SARS-CoV-2 spike (B and D) caused by individual sera from the indicated age and disease group. Each symbol is an individual healthy control or patient. Antibody levels to SARS-CoV-2 spike in MIS-C patients are plotted on a different scale from the rest. Red and blue numbers within the plots denote the p values of statistically significant increases and decreases, respectively, when comparing each disease group with the respective healthy control of the same age group. The older control group was also compared with the younger control group.

Article Snippet: pcCMV3- HCoV-OC43_spike , SinoBiological , Cat# VG40607-CF.

Techniques: Expressing

Journal: Med (New York, N.y.)

Article Title: Favorable antibody responses to human coronaviruses in children and adolescents with autoimmune rheumatic diseases

doi: 10.1016/j.medj.2021.08.001

Figure Lengend Snippet: SARS-CoV-2 neutralizing antibodies in pediatric and adolescent JIA, JDM, JSLE, and MIS-C patients (A) SARS-CoV-2-neutralizing antibody titers in the indicated age and disease group. Only patients with SARS-CoV-2 spike-binding antibodies detectable by flow cytometry were included. JIA, JDM, and JSLE patients of both age groups combined were compared with MIS-C patients by ANOVA on ranks tests. (B) Correlation of SARS-CoV-2-neutralizing antibody titers with levels of flow-cytometry-detectable antibodies to SARS-CoV-2 spike (left) or HCoV-OC43 spike (right). In (A) and (B), each symbol is an individual patient. In (B), one JSLE patient was removed from the regression analysis as an outlier for the HCoV-OC43 spike antibodies, based on the Kurtosis coefficient of the group.

Article Snippet: pcCMV3- HCoV-OC43_spike , SinoBiological , Cat# VG40607-CF.

Techniques: Binding Assay, Flow Cytometry

Journal: Med (New York, N.y.)

Article Title: Favorable antibody responses to human coronaviruses in children and adolescents with autoimmune rheumatic diseases

doi: 10.1016/j.medj.2021.08.001

Figure Lengend Snippet: Antibodies to coronaviral nucleoproteins in pediatric and adolescent JIA, JDM, JSLE, and MIS-C patients and age-matched controls (A–C) Mirror plots of the specific MFI increase of HEK293T cells expressing HCoV-OC43 nucleoprotein (top) or SARS-CoV-2 nucleoprotein (bottom) caused by individual sera. Each bar is an individual child or adolescent healthy control or JIA, JDM, or JSLE patient (A), MIS-C patient (B), or adult healthy control (C). Samples are plotted according to the signal of antibodies to the HCoV-OC43 nucleoprotein and in the same position in the mirror plots. (D) Correlation of the proportion of total HCoV-OC43 nucleoprotein-binding antibodies represented by IgM (top) or IgG (bottom) classes, and the age of the donor or patient. Each symbol is an individual sample.

Article Snippet: pcCMV3- HCoV-OC43_spike , SinoBiological , Cat# VG40607-CF.

Techniques: Expressing, Binding Assay

Journal: Med (New York, N.y.)

Article Title: Favorable antibody responses to human coronaviruses in children and adolescents with autoimmune rheumatic diseases

doi: 10.1016/j.medj.2021.08.001

Figure Lengend Snippet: Antibodies to coronaviral nucleoproteins in pediatric and adolescent JIA, JDM, JSLE, and MIS-C patients and controls of different ages Specific MFI increase of HEK293T cells expressing HCoV-OC43 nucleoprotein (A and C) or SARS-CoV-2 nucleoprotein (B and D) caused by individual sera from the indicated age and disease group. Each symbol is an individual healthy control or patient. Red and blue numbers within the plots denote the p values of statistically significant increases and decreases, respectively, when comparing each disease group with the respective healthy control of the same age group. The older control group was also compared with the younger control group.

Article Snippet: pcCMV3- HCoV-OC43_spike , SinoBiological , Cat# VG40607-CF.

Techniques: Expressing

Journal: Med (New York, N.y.)

Article Title: Favorable antibody responses to human coronaviruses in children and adolescents with autoimmune rheumatic diseases

doi: 10.1016/j.medj.2021.08.001

Figure Lengend Snippet: Ratios of the levels of antibodies to coronaviral spikes and nucleoproteins in pediatric and adolescent JIA, JDM, and JSLE patients and age-matched controls (A) The log2-transformed ratios of total antibodies to the HCoV-OC43 spike to total antibodies to the HCoV-OC43 nucleoprotein (S: N) are plotted for the indicated age and disease group. Each symbol is an individual sample. Numbers within the plots denote the p values of statistically significant increases, when comparing each disease group with the respective healthy control of the same age group. (B) Heatmap of ranked S: N ratios in the same samples, with each column representing a patient or control. The sample annotations for disease; age; disease activity; and treatment with steroids, biologics, or disease-modifying anti-rheumatic drugs (DMARDs) are also indicated.

Article Snippet: pcCMV3- HCoV-OC43_spike , SinoBiological , Cat# VG40607-CF.

Techniques: Transformation Assay, Activity Assay

Journal: Med (New York, N.y.)

Article Title: Favorable antibody responses to human coronaviruses in children and adolescents with autoimmune rheumatic diseases

doi: 10.1016/j.medj.2021.08.001

Figure Lengend Snippet:

Article Snippet: pcCMV3- HCoV-OC43_spike , SinoBiological , Cat# VG40607-CF.

Techniques: Recombinant, Software

Journal: Nature

Article Title: PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection

doi: 10.1038/s41586-023-06322-y

Figure Lengend Snippet: a , b , Quantification of viral entry efficiency in Huh7.5 cells inoculated with pseudoviruses bearing fusion proteins from HCoV-229E ( n = 5), HCoV-OC43 ( n = 3), hCoV-NL63 ( n = 3), hCoV-HKU1 ( n = 3), EBoV (n = 3) and HCV ( n = 5). EBoV: Ebola virus, HCV: Hepatitis C virus. c , Left, relative amount of intracellular viral RNA in Huh7.5 cells infected with DENV (Dengue virus type I) at an MOI = 0.5 for 24 h. The amount of viral RNA in NC cells was normalized to 1. ( n = 4) Right: Quantification of % infected cells in HeLa cells infected with HSV-1 VP26-GFP at an MOI of 0.1 for 48 h. d , Quantification of the relative entry efficiency of the indicated pseudovirus in Huh7.5 cells overexpressing (OE) PLSCR1 or IFITM3. The luminescence intensity in vector group was normalized to 1. n = 4. e , Schematic showing the dissection of the cell entry route of SARS-CoV-2. f , g , Effect of the indicated compounds on SARS-CoV-2 entry in Huh7.5 cells (MOI = 1, 48 hpi, n = 3) ( f ) and A549-ACE2 cells (MOI = 0.2, 24 hpi, n = 3) ( g ). E-64d: 20 μM, Camostat: 30 μM, Bfa (Brefeldin a): 10 μM, HCQ: 10 μM. Cells were treated with indicated compounds 2 h before infection. h , Quantification of SARS-CoV-2 infection in E-64d (20 μM) treated or untreated Huh7.5 cells overexpressing vector or PLSCR1 with or without ectopic expression of TMPRSS2 (MOI = 1, 48 hpi). ( n = 4) i , Left, dose response of indicated compounds on SARS-CoV-2 infection in Calu-3 (MOI = 1, 24 hpi, n = 4). The amount of viral RNA in DMSO group was normalized to 1. E64-d and Camostat groups share the same DMSO control group. Right, quantification of SARS-CoV-2 infection in Control or PLSCR1 -KO Calu-3 (MOI = 1, 24 hpi, n = 4) treated with indicated compounds (E-64d: 20 μM, Camostat: 20 μM). Cells were treated with the indicated compounds 2 h before infection. The amount of viral RNA in NC-DMSO group was normalized to 1. Data are mean ± s.d. P values were calculated using one-way ANOVA followed by Tukey’s multiple comparison test in a – c , d (HCoV-NL63 group), f , g , Brown–Forsythe and Welch ANOVA with Dunnett’s post-hoc test in d (SARS-CoV-2 and EBoV group), two-sided Student’s t -test in h , two-way ANOVA followed by Tukey’s multiple comparison test in i (left) or two-way ANOVA followed by Šídák’s multiple comparisons test in i (right). Experiments in this figure were performed three times.

Article Snippet: Expression plasmids of the glycoproteins for HcoV-229E (VG40605-UT), HcoV-OC43 (VG40607-UT), HcoV-HKU1 (VG40021-UT) and EboV (VG40304-CF) were purchased from Sino Biological. pLV-EF1α-Flag-IRES-Hygro was modified from pLV-EF1α-IRES-Hygro by inserting a Flag tag between the promoter region and the multiple cloning site.

Techniques: Infection, Plasmid Preparation, Dissection, Expressing

Journal: eLife

Article Title: Murine alveolar macrophages rapidly accumulate intranasally administered SARS-CoV-2 Spike protein leading to neutrophil recruitment and damage

doi: 10.7554/eLife.86764

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , Human coronavirus (HCoV-229E) Spike Gene ORF cDNA clone expression plasmid (Codon Optimized) HCoV-229E , SinoBiological , VG40605-UT , .

Techniques: Blocking Assay, Imaging, Staining, Recombinant, Plasmid Preparation, Expressing, Luciferase, Cell Culture, Transfection

Journal: bioRxiv

Article Title: ACE2 engagement exposes the fusion peptide to pan-coronavirus neutralizing antibodies

doi: 10.1101/2022.03.30.486377

Figure Lengend Snippet: Binding of anti-fusion peptide mAbs (8 μg/ml) to 293T transiently co-transfected with plasmids encoding ZsGreen and SARS-CoV-2 S (293T-S) and SARS-CoV-2 S-2P (293T-S-2P), SARS-CoV Δ19 S, MERS-CoV S, NL63 S and 229E S, in the presence or absence of receptors ACE2 (27 μg/ml) for SARS-CoV-2 and SARS-CoV S, DPP4 (27 μg/ml) for MERS-CoV S, and APN (27 μg/ml) for 229E S, as measured by flow cytometry. * p < 0.05, ** p < 0.01, **** p < 0.0001 Ratio paired t test.

Article Snippet: For transient expression of HCoV spike proteins, 293T cells were co-transfected, with plasmid encoding ZsGreen (Bei Resources, catalog no. NR-52516) and corresponding HCoV spike proteins: SARS-CoV-2 Wuhan-Hu-1 S (catalog no. NR-52514) from Bei Resources; MERS-CoV S (VG40069-G-N), 229E S (VG40605-UT), NL63 S (VG40604-UT) from SinoBiological; SARS-CoV S (VG40150-G-N) from SinoBiological that was cloned into pHDM expression plasmid with 19 amino-acid C-terminal truncation , using PEI in Optimem as above.

Techniques: Binding Assay, Transfection, Flow Cytometry